Western Blot analysis of whole cell lysates probed with (A) ERK1/2 antibody or (B) ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody (VMA00713) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000. Membranes were treated with (+) and without (-) Lambda Protein Phosphatase as indicated and visualized on the ChemiDoc MP with (A) 40 second or (B)15 second exposure. Arrow points to ERK1 (pThr202)/ERK2 (pTyr204) (molecular weight 42 & 44 kDa).

Western Blot analysis of whole cell lysates probed with (A) ERK1/2 antibody or (B) ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody (VMA00713) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) Lambda Protein Phosphatase as indicated and visualized on the ChemiDoc MP with (A) 40 second or (B)15 second exposure. Arrow points to ERK1 (pThr202)/ERK2 (pTyr204) (molecular weight 42 & 44 kDa).